HISTOCHEMISCHE DIFFERENZIERUNG VERSCHIEDENER ZELLEN IN DER HAUPTSPEICHELDRÜSE VON MYZUS PERSICAE

Abstract

In der Hauptspeicheldrüse von Myzus persicae wurden mit histochemischen Nachweismethoden vier Zellgruppen bestimmt. Diese Zellgruppen ließen sich fluoreszenzmikroskopisch auf Grund ihres Bindungsvermögens für Acridinorange unterscheiden, außerdem durch ihren Gehalt an RNS und verschiedenen Proteinen sowie durch ihren Gehalt an Kohlenhydraten (PAS‐Reaktion), Hyaluronsäure und anderen Hale‐positiven Substanzen. Die Speichelscheide reagierte auf Nachweise für Kohlenhydrate und Protein positiv.SummaryHISTOCHEMICAL DIFFERENTIATION OF SEVERAL CELL GROUPS OF THE PRINCIPAL SALIVARY GLAND OF MYZUS PERSICAEFixed and sectioned principal salivary glands of Myzus persicae stained with acridine orange at different pH‐values (pH 2.6 − 7.0) were investigated under the fluorescence microscope. There were 4 groups of cells, which differed in their ability to absorb acridine orange (Abb. 1). By histochemical demonstration of different substrates a characterization of those 4 groups was possible: Cells A and F (see Abb. 1) contain much RNA and also much protein, which is distinguished by a high cystine content. Cells B, C and I contain much RNA and protein, but reactions for disulphide groups (cystine) were negative. There was an additional high content of hyaluronic acid. Cells D and E. Besides RNA and protein, PAS‐positive and Hale‐positive substances were found. Because of the different distribution of the latter two substances in the cell different polysaccharides are assumed. Cells G and H. In the G‐cells no considerable amounts of RNA, protein and polysaccharides were found. In the H‐cells only a weak PAS‐reaction took place. Probably these cells produce no secretion for the saliva. The stylet sheath was Hale‐ and PAS‐positiv; thus the presence of protein could be demonstrated.