Abstract
Comparing techniques already tested with helminths, and some as yet untested, for light and electron microscope histochemistry, it was found that: 1. 1. The epicuticle of Hymenolepis citelli, Haematoloechus medioplexus and Moniliformis dubius is composed of acid mucosubstance. The epicuticle of M. dubius is a strongly acidic, sulfated acid mucosubstance, whereas the nature of the epicuticle mucosubstance of the other two worms is uncertain. 2. 2. The protein composition of the epicuticle of the three worms ranges from acid to neutral. 3. 3. No acid mucosubstance was found in the trematode gut mucosa which is composed of weakly basic protein, and probably regions of neutral mucosubstance.
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