Abstract
A rapid technique for the histochemical localization of cysteine-rich proteins in plant tissues was developed. It is based on the immediate transfer of proteins to nitrocellulose membranes when a fresh cut organ is pressed against the membrane surface. The print was labeled for cysteine-rich proteins by reduction and alkylation of cysteinyl residues with dansylated iodoacetamide [ N-iodoacetyl- N′-(-5-sulfo-1-naphthyl)ethylenediamine]. The S-carboxymethylated proteins were visualized by their fluorescence when excited with 360 nm light.
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