Abstract

The role of granular leucocytes, particularly neutrophils, during wound healing in the injured rabbit lens and the distribution of acid phosphatase were investigated using electron microscopic histochemistry. Granular leucocytes first appeared in the wound coagulum approximately after 12 h following injury. They contained granules which gave a positive reaction for acid phosphatase. At 12 and 24 h acid phosphatase localization was found to be extracellular and was most pronounced at the apical region of the epithelial cells. This localization was most intense at the immediate periphery of the wound and decreased with distance from the site of injury. At 48 h following injury this extracellular localization decreased. The possible role of hydrolytic enzymes in tissue repair is discussed.

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