Histochemical Evaluation of Prostate Diseases: Integrating Bacterial Infections and Cancer Subtyping

Abstract

Background: Prostate diseases, including bacterial infections and prostate cancer, pose significant health concerns worldwide. However, limitedresearch has focused on the comprehensive characterization of bacterial infections in prostate diseases. This study aimed to address these knowledge gaps by investigating the prevalence of bacterial infections in prostate diseases as well as prostate cancer subtypes. Methods: A retrospective study using formalin-fixed paraffin-embedded (FFPE) prostate biopsy tissue samples from patients diagnosed with prostatediseases. Relevant clinical information was collected, and Gram staining was performed to detect the presence of Gram-negative bacteria. Southgate's mucicarmine staining was utilized to categorize prostate cancer subtypes. Data were analyzed using SPSS version 20.0. Results: Among the 50 patients included in the study, the majority (48.0%) fell within the age range of 60-69 years. Prostatic hyperplasia was the mostprevalent prostate disease (70.0%), followed by prostate cancer (28.0%). Gram-negative bacteria were identified in 56% of the FFPE. Categorization of prostate cancer subtypes using mucicarmine staining revealed that 71.4% of FFPE exhibited a positive reaction, indicating the presence of mucin. Conclusion: This study has identified bacterial infections in prostate diseases, focusing on Gram-negative bacteria, mucin positive prostate cancer subtype using mucicarmine staining were as well identified.