Abstract
The article presents the data of study of the effectiveness of various combinations of histochemical staining to determine the co-location of mast cells with a fibrous component of the connective tissue of Wistar line laboratory rats skin. The best results are obtained with combination protocols such as metachromatically detection of mast cells in combination with the impregnation with silver or picrofuchsin, brilliant green coloring with the methodology of Van Gieson or picrofuchsin, and aniline blue neutral red. The proposed variants of histochemical protocols can be used to analyze the participation of mast cells in the remodeling of the cell matrix of tissue microenvironment in the development of adaptive and pathological processes. The obtained results contribute to the expansion of currently available scientific ideas about the laws of the process of fibrillogenesis in the extracellular matrix and provide new data on the targeted participation of the components of the mast cell secretome in the molecular mechanisms of fiber formation.
Highlights
The article presents the data of study of the effectiveness of various combinations of histochemical staining to determine the co-location of mast cells with a fibrous component of the connective tissue of Wistar line laboratory rats skin
The best results are obtained with combination protocols such as metachromatically detection of mast cells in combination with the impregnation with silver or picrofuchsin, brilliant green coloring with the methodology of Van Gieson or picrofuchsin, and aniline blue neutral red
The proposed variants of histochemical protocols can be used to analyze the participation of mast cells in the remodeling of the cell matrix of tissue microenvironment in the development of adaptive and pathological processes
Summary
ФГБОУ ВО «Воронежский государственный медицинский университет им. Н. В статье представлены данные проведенного исследования эффективности различных комбинаций гистохимического окрашивания для выявления солокализации тучных клеток с волокнистым компонентом соединительной ткани кожи лабораторных крыс линии Wistar. Предложенные варианты гистохимических протоколов могут быть использованы для анализа участия тучных клеток в ремоделировании межклеточного матрикса тканевого микроокружения при развитии адаптивных и патологических процессов. The obtained results contribute to the expansion of currently available scientific ideas about the laws of the process of fibrillogenesis in the extracellular matrix and provide new data on the targeted participation of the components of the mast cell secretome in the molecular mechanisms of fiber formation. Пионерские работы основоположников гистохимического направления в морфологии для выявления селективных компонентов межклеточного матрикса положили начало большому количеству исследований по детекции коллагеновых и эластических волокон, а также определению качественного состава аморфного компонента [4, 6, 9, 15]. Т. 7, No 3 ◊ Journal of anatomy and histopathology
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