Abstract
Objective: To demonstrate the mucin changes in Ulcerative Colitis, to evaluate the benefit of the staining method and to demonstrate the CEA staining pattern in dysplasia complicating ulcerative colitis (U.C). Methods: Colorectal biopsies were examined for changes of U.C for which a combined PAS/Alcian blue stain was applied. CEA immunohistochemical stain was used for cases of dysplasia complicating ulcerative colitis. Results: One hundred colorectal biopsies were examined, U.C. was diagnosed in (22%) of cases, the mean age was (36.68) years, (13) were males, and (9) were females. The prevalence of dysplastic changes complicating U.C were detected in (22.7%).The rectum was the most frequent site of dysplasia complicating, U.C.. Two of the cases showed mild dysplastic changes; two showed moderate dysplasia, and only one showed severe dysplasia. The secretory activity of mucin in colorectal mucosa was tow in surface epithelium and varied in the crypts from absent or weak to moderate reduction. The site of CEA distribution seemed to be affected mainly by degree of dysplasia, it was predominantly along the apical surface of the cells and also in the cytoplasm in case of mild dysplasia, while in moderate dysplasia it was of cytoplasmic distribution, and in severe dysplasia there was intensive cytoplasmic distribution. Conclusion: There was an increase in relative frequency of U.C. Immunohistochemical study of CEA localization in dysplastic gland is helpful in detection of early malignant change in U.C.
Highlights
One hundred colorectal biopsies were examined, U.C. was diagnosed in (22%) of cases, the mean age was (36.68) years, (13) were males, and (9) were females
Colorectal biopsies were collected prospectively from (100) non selected patients with symptoms of large bowel disorders examined at the endoscopic units of AL-Jamhhori Teaching Hospital, AL-Salam Hospital, and private hospitals, in the period from October 2001 to July 2002.The age ranged from (2) months to (80) years
Colonoscopy and the detection of dysplasia in patients with longstandingU.C. The lancent, 1980; 20: 620-621. [Thesis] 2000 GIT center College of Medicine, Baghdad University. U.C. in the predictive value of low grade dysplasia Gastroentrology, 1992; 103(2): 431-8. (Cited by). 33.Suskin K, Muto J, Agawa S, Nippen S, Cakkai Z. Dysplasia in U.C. detected by surveillance colonoscopy 1991: 28 (4): 1051-9 (Abstract)
Summary
Colorectal biopsies were collected prospectively from (100) non selected patients with symptoms of large bowel disorders examined at the endoscopic units of AL-Jamhhori Teaching Hospital, AL-Salam Hospital, and private hospitals, in the period from October 2001 to July 2002.The age ranged from (2) months to (80) years. All biopsies were fixed immediately in (10%) formaldehyde for (24) hours, the samples were processed routinely and embedded in paraffin blocks and stained with Haematoxylin and Eosin (H&E) stain. Immunohistochemical staining for CEA was performed in cases of U.C to detect dysplastic changes by using an improved Biotin- streptavidin Amplified (BSA) detection system. The staining procedure was done according to the manufacturer instructions of staining protocol (16). Statistical methods: The statistical evaluation was performed using mean, range, and chi square test
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
