Abstract
Organophosphorous pesticide sumithion, the O, O Dimethyl O- (3-methyl-4-nitrophenyl), is a widely used pesticide in agricultural land and in aquaculture to control some harmful pests. A study was conducted in laboratory condition with aquaria (36 inch × 10 inch × 12 inch) to evaluate the effects of sumithion on histo-architecture of intestine in zebrafish (Danio rerio). The experiment was carried out with three treatments (T1: 0.5 ppm, T2: 1.0 ppm, T3: 2.0 ppm) and a control (T0: 0ppm), each having three replications. Zebra fishes (Danio rerio) (4±1cm and 0.9±0.2g) were stocked for the experiment and sacrificed after 7 days of exposure of sumithion. During the study period, the temperature was almost constant (21- 22OC) but dissolved oxygen, pH and total alkalinity values were tended to decrease with the increase in concentrations of test chemicals. The histo-architectural changes in intestine suggested that the intestinal epithelial cells, lumen, villi and intestinal folding were varied significantly (P<0.05) in treatment groups rather than the control groups (T0). Disappeared mucosa (DM) along with abnormal lumen (AL) were found in case of T1, while destructed intestinal villi (DV), sloughing of superficial epidermal cells (SEC) and uneven intestinal folding (UF) were found in T2 and T3. The obtained result supports the toxic potentiality of sumithion. Therefore, the use of sumithion must be evaluated carefully in agriculture and aquaculture.Res. Agric., Livest. Fish.2(3): 499-506, December 2015
Highlights
Pesticides have been considered as a major threat of gradual degradation for the aquatic ecosystem (Salam et al, 2015; Sharmin et al, 2015)
No mortality was observed in control treatment, whereas the mortality percentage increased as the concentration of sumithion increased
The current research activity was conducted on the basis of an aim to evaluate the effects of sumithion on histo-architecture of intestine in zebrafish
Summary
Pesticides have been considered as a major threat of gradual degradation for the aquatic ecosystem (Salam et al, 2015; Sharmin et al, 2015). An organophosphate insecticide is considered somewhat toxic to fish (Thomson, 1989) but no clear evidence on the intestinal morphological alteration was found so far. Toxicology studies on different tissues (Thophon et al, 2003) can provide information about tissues injuries and damages of organs resulting in morphological dysfunction. Being one of the most common, small and robust vertebrate model organisms, zebrafish got the highest priority to conduct this experiment (Shahjahan et al, 2013). In adverse condition, different organs such as intestine, gonad etc. This study was carried out to determine the effect of sumithion on intestine of zebrafish
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