Abstract
The procedure for determining histidase activity in stratum corneum has been modified with respect to thiol protective reagent, reaction pH, substrate concentration, and estimation of product. Stratum corneum from 46 control subjects, 5 histidinemic patients, 7 parents, and 5 sibs were assayed for histidase activity. The mean value for the parents was one-half that for control subjects; all affected individuals had very low values. Although a gene-dosage relationship can be demonstrated, the usefulness of stratum corneum histidase activity has limitations for diagnosis and for heterozygote identification. The three genotypes cannot be clearly separated, local medication may reduce enzyme activity, genetic variants may not be detected, and differences in activity are found between samples from the feet and hands.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
