Abstract
Objective To investigate the role of class II histidine deacetylase inhibitor (HDACIs) Trichostatin A (TSA) in inhibiting corneal graft rejection. Methods The inhibition rate of TSA on effector T cells and the ratio of TSA-induced regulatory T cells were evaluated with proliferation inhibition assay and Treg cell induction assay, and the survival rate of corneal grafts after TSA treatment was observed. Results The proliferation rates of cells exposed to 1 ng/ml, 5 ng/ml, and 10 ng/ml TSA in the in vitro proliferation inhibition test were (74.60±0.03)%, (26.27±0.01)%, and (25.64±0.03)%, respectively. The ratio of CD25+ Foxp3+ cells exposed to 5 ng/ml and 20 ng/ml TSA in vitro was (3.19±1.32)% and (7.73±1.52)%. In vivo experiment, the survival rate of corneal grafts was the highest in TSA treatment group. Conclusion The survival rate of mouse corneal grafts is improved after TSA treatment. Key words: Histone deacetylase inhibitors; Effector T cells; Regulatory T cells; Corneal transplantation
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Chinese Journal of Ocular Trauma and Occupational Eye Disease
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
