Abstract
Histamine H 1-receptors in membranes of the various mamalian retinas were studied by [ 3H]mepyramine binding assay. Specific [ 3H]mepyramine bindings to bovine, pig, dog and human retinas were observed with the dissociation constants (K D), 3.8±1.2 nM, 1.8±0.6 nM, 2.6±0.6 nM and 3.0±0.9 nM, respectively, which were similar to those found in brains. But there was no detectable specific binding in the guinea-pig and rabbit retinas. The number of binding sites (Bmax) ranged from negligible value to 290.7±51.7 fmole/ mg protein (human retina). Some H 1-antagonists acted as potent agents in competing with [ 3H]mepyramine binding to bovine and pig retinas. These results indicated that histamine H 1-receptors exist in some mammalian retina and have similar characteristics to those in brain membranes, but they distributes in the wide difference of the binding capacities among the species, while in brain variations were smaller.
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