Histamine functionalized magnetic nanoparticles (HIS-MNP) as a sorbent for thin film microextraction of endocrine disrupting compounds in aqueous samples and determination by high performance liquid chromatography-fluorescence detection

Abstract

This paper describes the development of a new method in which histamine-functionalized magnetic nanoparticles (HIS-MNP) are used as the extraction phase associated with thin-film microextraction and a 96-well plate system. This approach was applied to the determination of estrone, estriol, 17-β-estradiol, 17α-ethynylestradiol, 4-octylphenol and 4-nonylphenol in aqueous samples by HPLC-FLD. Extraction and liquid desorption steps were optimized employing multivariate and univariate procedures. The optimum extraction conditions were 50 min of extraction time, no salt addition and sample pH 5. Liquid desorption was carried out for 30 min with 300 μL of acetonitrile:methanol (50:50 v/v). The quantification limits varied between 0.5 and 20 μg L−1. The method showed recovery values between 82 and 120%, and relative standard deviations varied from 0.1 to 13.6%.