Histamine content and histamine-forming bacteria in mahi-mahi (Coryphaena hippurus) fillets and dried products

Abstract

Forty-two mahi-mahi fillets and 17 dried products sold in retail markets in Taiwan were tested for histamine and histamine-forming bacteria. The levels of pH, salt content, water content, Aw, TVBN, APC, TC and Escherichia coli in mahi-mahi fillet samples ranged from 5.6 to 6.5, 0.05 to 2.44%, 70.9 to 82.8%, 0.95 to 0.99, 5.9 to 23.5 mg/100 g, 3.1 to 7.0 log CFU/g, <3 to 1650 MPN/g and <3 to 45 MPN/g, respectively. The levels of pH, salt content, water content, Aw, TVBN, APC, TC and E. coli in dried mahi-mahi samples ranged from 5.7 to 6.4, 0.63 to 20.13%, 7.1 to 42.9%, 0.51 to 0.85, 21.4 to 133.9 mg/100 g, 3.6 to 8.7 log CFU/g, <3 to 5900 MPN/g and <3 to 2500 MPN/g, respectively. The average content of various biogenic amines in fillets samples was less than 0.3 mg/100 g. Four of the 17 dried samples (23.4%) had histamine levels greater than the FDA guideline of 5 mg/100 g for scombroid fish and/or product with one of them containing 68.15 mg/100 g of histamine, which is greater than the 50 mg/100 g hazard action level. Eight histamine-producing bacterial isolates, capable of producing 12.6 ppm–562 ppm of histamine in trypticase soy broth supplemented with 1.0% l-histidine (TSBH), were identified as Raoultella ornithinolytica (three isolates), Pantoea agglomerans (two isolates), Proteus vulgaris (two isolates) and Enterobacter amnigenus (one isolate), by 16S rDNA sequencing with PCR amplification.