Abstract
Histamine is an immune mediator that is mainly secreted when an immediate, rapid response is needed in the body, and an excessive secretion of histamine or lack of enzymes that degrade histamine can result in various side effects. Histamine binding protein (HBP) is secreted by a mite species to prevent the host’s histamine-induced immune responses by binding the histamine molecule in the blood. Cloning was performed to express HBP on the yeast surface (MBTL-GWL-1), and immunofluorescence (IF) and western blot was performed to confirm the expression of the recombinant protein. The histamine inhibitory ability of GWL-1 cells was tested according to the cell concentration. The highest inhibitory ability of 1.30 × 107 CFU/ml of GWL-1 cells was of about 60 %. The GWL-1 cell concentration and the degree of histamine inhibition were confirmed to be dose-dependent, and dead cell debris was shown to have a histamine inhibitory effect, although not as much as that of whole cells. Phagocytosis assays were performed to determine whether histamine affected the RAW 264.7 cell’s phagocytosis, and to indirectly confirm the GWL-1 cell’s histamine inhibition. By confirming that, we found that GWL-1 captures histamine. Therefore, it can be expected to become a competitive material in the anti-allergy market.
Highlights
Histamine [2-(4-imidazolyl)-ethylamine] is an immune mediator that is secreted mainly when an immediate, rapid response is needed in the body
Construction of the recombinant yeast Female-specific histamine-binding protein 2 derived from Rhipicephalus appendiculatus was expressed on the yeast surface
To compare the effect on the recombinant cells, GWL-0 that excluded only the Histamine binding protein (HBP) gene from GWL-1’s vector and a Mock with only the pYES2 vector were used as controls
Summary
Histamine [2-(4-imidazolyl)-ethylamine] is an immune mediator that is secreted mainly when an immediate, rapid response is needed in the body. If histamine is normally regulated, it is not a problem, but an excessive secretion of histamine or lack of enzymes that degrade histamine can result in itching and inflammatory reactions (Schneider et al 2010). Skin diseases that are caused by the action of uncontrolled histamine, such as atopy and psoriasis, are often a problem because this reaction mediates the vasodilatation and skin nerve stimulation that cause itching (Werfel et al 2016). Antihistamines are widely used to prevent various allergic reactions by binding to histamine receptors and suppressing the immune response caused by histamine (Dykewicz et al 1998). Jang et al AMB Expr (2021) 11:146 antihistamines often cause side effects in the central nervous system including drowsiness, fatigue, memory loss, and attention deficit, as well as digestive disorders such as constipation diarrhea, nausea, and vomiting (Hindmarch and Shamsi 1999)
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