Hispidin attenuates bleomycin-induced idiopathic pulmonary fibrosis via an anti-oxidative effect in A549 cells

Chen-Xi Ren,Taeho Kwon,Dan-Ping Xie,Xiao-Yu Guo,Xin Jin,Hu-Nan Sun,Yu-Dong Cui,Li-Yun Yu

doi:10.1186/s13765-021-00646-x

Abstract

Idiopathic pulmonary fibrosis (IPF) is a serious and irreversible chronic lung disease. Bleomycin (BLM) is an anticancer drug, which can cause severe lung toxicity. The main target of oxidative stress-induced lung injury is alveolar epithelial cells, which lead to interstitial fibrosis. The present study investigated whether hispidin (HP), which has excellent antioxidant activity, attenuates bleomycin-induced pulmonary fibrosis via anti-oxidative effects in A549 cells. We found that hispidin reduced bleomycin-induced fibrosis of A549 cells by reducing reactive oxygen species (ROS) levels and inhibiting epithelial-mesenchymal transition. Taken together, our data suggest that hispidin has therapeutic potential in preventing bleomycin-induced pulmonary fibrosis.

Highlights

Idiopathic pulmonary fibrosis (IPF) is a serious and irreversible chronic lung disease [1]
In light of the above-mentioned gaps in the field, this study aimed to explore the preventive and therapeutic effects of hispidin on pulmonary fibrosis.First, bleomycin was used to induce pulmonary fibrosis, and A549 cells were pretreated with HP
BLM inhibits proliferation and promotes fibrosis in A549 cells We constructed a cell model of alveolar epithelial cell fibrosis in vitro and used BLM was to induce the fibrosis of A549 human alveolar epithelial cells

Summary

Introduction

Idiopathic pulmonary fibrosis (IPF) is a serious and irreversible chronic lung disease [1]. IPF has the following characteristics: Excessive accumulation of fibroblasts, extensive deposition of the extracellular matrix, alveolar structure damage, and a gradual decline of lung function [2]. Sawdust, sand, and silica exposure may lead to repeated injury of alveolar epithelial cells. Many studies have shown that injury and apoptosis of alveolar epithelial type II cells are important early features of IPF [3]. IPF is accompanied by epithelial cell apoptosis, epithelial-mesenchymal transition (EMT) and matrix. Bleomycin (BLM) is a complex glycopeptide with antitumor properties. It is often used as an antibiotic to treat various cancers in the clinic. After bleomycin reaches the lung tissue, it leads to the production of reactive oxygen species (ROS) under the

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