Abstract
This study was conducted to investigate the purification methods for high-yield of α-linolenic acid (ALA) from Perilla frutescens var. japonica oil (PO). PO was treated with 2 g of urea, cooled for 24 h, and 81.75 % of ALA was obtained after gas chromatography–flame ionization detector analysis. Urea complexation at low temperature (refrigeration) is a prospective method for the extraction of unsaturated fatty acids in high yields. Our results suggest that urea treatment with cooling at 10 °C could be used for purification of ALA from PO. It will be enhanced for the development of nutritional, medical, and cosmetic value.
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