Abstract
Polygoni Multiflori Radix Praeparata (PMRP), as the processed product of tuberous roots of Polygonum multiflorum Thunb., is one of the most famous traditional Chinese medicines, with a long history. However, in recent years, liver adverse reactions linked to PMRP have been frequently reported. Our work attempted to investigate the chemical constituents of PMRP for clinical research and safe medication. In this study, an effective and rapid method was established to separate and characterize the constituents in PMRP by combining ultra-high performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS). Based on the accurate mass measurements for molecular and characteristic fragment ions, a total of 103 compounds, including 24 anthraquinones, 21 stilbenes, 15 phenolic acids, 14 flavones, and 29 other compounds were identified or tentatively characterized. Forty-eight compounds were tentatively characterized from PMRP for the first time, and their fragmentation behaviors were summarized. There were 101 components in PMRP ethanol extract (PMRPE) and 91 components in PMRP water extract (PMRPW). Simultaneously, the peak areas of several potential xenobiotic components were compared in the detection, which showed that PMRPE has a higher content of anthraquinones and stilbenes. The obtained results can be used in pharmacological and toxicological research and provided useful information for further in vitro and in vivo studies.
Highlights
Polygoni Multiflori Radix Praeparata (Zhiheshouwu in Chinese, PMRP), as a processed root of Polygonum multiflorum Thunb., has a long history in clinical application
The results indicated that the UHPLC-Q-Exactive Orbitrap-MS developed in this study is appropriate to detect the chemical constituents in PMRP
The results indicated tha3totfh1e9 UHPLC-Q-Exactive Orbitrap-MS developed in this study is appropriate to detect the chemical constituents in PMRP
Summary
Polygoni Multiflori Radix Praeparata (Zhiheshouwu in Chinese, PMRP), as a processed root of Polygonum multiflorum Thunb. (heshouwu in Chinese, PMR), has a long history in clinical application. The common processing method of PMRP is steaming or boiling PMR with a black bean decoction, as prescribed by the Chinese Pharmacopoeia. Modern research has revealed that anthraquinones, stilbenes, flavonoids, and phenolic acids in PMRP are the major compounds of its pharmacological activities [5,6]. Several polyhydroxy stilbenes such as 2,3,5,4 -tetrahydroxystilbene-2-Oβ-D-glucoside(THSG) have a similar structure to resveratrol, and they have been proven to have a strong ability to antioxidize and perform free radical scavenging activities [7]. Three anthraquinones, including physcion, emodin, and questin, were regarded as Cdc25B phosphatase inhibitors by strongly inhibiting the growth of human colon cancer cells [10]. Proanthocyanidins, isolated from MPRP, have the potential to be functional ingredients in reducing postprandial hyperglycemia, by inhibiting α-amylase and α-glucosidase [11]
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