High-Speed Three-Dimensional Microscope for Dynamically Moving Biological Objects Based on Parallel Phase-Shifting Digital Holographic Microscopy

Abstract

We succeeded in 3-D motion-picture imaging for dynamic motion of biological objects swimming at different depths by parallel phase-shifting digital holographic microscopy (PPSDHM). PPSDHM is a technique for high-quality instantaneous 3-D imaging of specimens in the microscopic field of view by single-shot recording of multiple phase-shifted holograms. We constructed a PPSDHM for recording high-speed dynamic phenomena and then motions of biological objects in water were recorded at 20 000 and 150 000 frames/s, respectively, which is the fastest among the previous reports on 3-D imaging of biological objects.