Abstract
In eukaryotic cell, various organelles and cytoskeletons are very dynamic, yet highly organized to orchestrate complex cellular functions. Visualizing these interactions requires noninvasive, long duration imaging of the intracellular environment at high spatial and temporal resolution. However, the tradeoffs between spatial and temporal resolution, and low phototoxicity/photobleaching in current super-resolution imaging techniques prevent biologists from accurately characterizing these dynamic processes. To achieve these normally opposing goals, in this talk I will discuss our latest developments in grazing incidence structured illumination microscopy (GI-SIM), and lattice light sheet microscopy (LLSM).
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