Abstract
Doppler Raman (DR) spectroscopy is a coherent Raman technique that combines impulsive Raman excitation with novel frequency shift detection to enable high-sensitivity Raman spectroscopy in the biological fingerprint region (500cm-1-1500cm-1) and the low frequency regime from 10cm-1 – 500cm-1. Using DR, we demonstrate nonresonant Raman spectroscopy on a suite of biologically significant targets involved in cell respiration including cytochrome c, adenosine triphosphate (ATP), flavin adenine dinucleotide (FAD), and nicotinamide adenine dinucleotide (NADH). High-sensitivity detection of low-to-medium frequency Raman vibrational modes may provide a tool to monitor states of cell respiration along with large molecular structural changes such as protein conformational dynamics.
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