Abstract
Laser-based wide-field coherent imaging methods suffer from low image contrast due to the speckle noise as well as poor lateral resolution using collimated illumination. To improve the image contrast and spatial resolution for label-free cell imaging applications, we propose a new dynamic speckle illumination scheme using perfect optical vortex (POV) beams that can provide finer speckles with more uniform distributions. The low spatial coherence from the POV speckle field has significantly improved the signal-to-noise ratio (SNR) and the image contrast, thus contributing to a high spatial resolution that matches the diffraction limit in our cell imaging experiments. Importantly, the depth-resolved imaging capability has been realized which has allowed us to visualize fine subcellular structures at different focal planes.
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