Abstract
The useful application of high-pressure liquid chromatography in biochemical research with adenine nucleotide analogues is demonstrated by using a glass-walled separating column at inlet pressures between 100 and 200 atm. Three different aspects are highlighted: (1) purity control of the adenine nucleotide analogues; (2) direct reaction monitoring by high-pressure liquid chromatography; (3) high-pressure liquid chromatography data and mononucleotide conformation.
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