Abstract
The effects of indole structure and eluent pH and composition on the retention of 22 indole derivatives on layers of Sil C 18-50 untreated or impregnated with 4% N-dodecylpyridinium chloride were evaluated. Indole derivatives with a carboxyl group in the molecule are highly retained in acidic solution and have minimum retention at strongly alkaline pH. The optimum coinditions for the separation of the greatest number of compounds were investigated and theoretical and experimental two-dimensional chromatograms (which allow the separation of 20 compounds) are reported. On home-made layers of ammonium tungstophosphate (AWP), solutions of ammonium nitrate at different concentrations were employed for the separation of those indole derivatives which exhibit similar characteristics on Sil C 18-50 plates. The AWP layers allow the determination of 5-hydroxyindole-3-acetic acid in urine at 2 mg per 24 h levels.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
