High-performance liquid chromatography ofα-amino acids on a polystyrene resin with fixed ligands of the type (R)-N′, N′-dibenzyl-1,2-propanediamine

Abstract

A cross-linked polystyrene resin with fixed ligands of the type (R)-N′, N′-dibenzyl-1,2-propanediamine in the form of a copper(II) complex displays high enantio-selectivity in ligand-exchange chromatography of amino acids. A microbore column (100 mm×1 mm i.d.) packed with angular particles of dp=5–10 μm generated up to 3500 theoretical plates, enabling a complete resolution of a mixture of three racemic amino acids into six components under isocratic conditions.