Abstract
Volatile solutions were applied as eluents in the hydroxyapatite high-performance liquid chromatography (HPLC) of proteins and the elution behaviour was investigated. Four standard proteins were loaded on a ceramic hydroxyapatite column for HPLC and eluted with linear gradients of seven volatile solutions. Hydroxyapatite HPLC using ammonium hydrogencarbonate solution seems to be the best alternative although the resolution and recovery of proteins were lower than those obtained with a phosphate buffer system. The system was applied to the purification of a monoclonal antibody and of an enzyme in small amounts.
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