Acquired immune deficiency syndrome or acquired immunodeficiency syndrome (AIDS or Aids) is symptoms and infections resulting from the damage to the human immune system. The purpose of this paper is to review the literature for high performance liquid chromatography methods to simultaneous determination of anti-retroviral drugs in biological matrices covering the articles mainly from 2001 to 2009. The biological matrix samples include various biological matrices such as human plasma, serum, breast milk, saliva, cerebrospinal fluid and seminal fluid. The review covers high-performance liquid chromatography coupled with ultraviolet detection and reversed-phase high-performance liquid chromatography coupled with tandem mass spectrometry.

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