Abstract
A rapid, sensitive and specific high performance liquid chromatography–electrospray ionization tandem quadrupole mass spectrometry (HPLC–MS/MS) method was developed and validated for the determination of 3- n-butylphthalide in rat plasma. Following protein precipitation with acetonitrile, 3- n-butylphthalide and glipizide (internal standard, I.S.) were separated using a gradient elution program on a C 18 column and detected by mass spectrometry in positive ion mode with the multiple reaction monitoring (MRM) mode using the respective precursor to product ion combinations of m/z 191/145 for 3- n-butylphthalide and m/z 446/321 for glipizide, respectively. The total chromatographic running time was 2.5 min. The method was linear over the concentration range of 11.14–3480.00 ng/mL, using as little as 100 μL plasma. The lower limit of quantification (LLOQ) was 5.57 ng/mL. Finally, the method was successfully used to support a preclinical pharmacokinetic study of 3- n-butylphthalide in rats following intravenous administration.
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