Abstract
Abstract The separation of 3-N-acetylglucosaminides of cholate, chenodeoxycholate, deoxycholate, ursodeoxycholate and litho-cholate, and their glycine- and taurine-conjugates has been carried out by high-performance liquid chromatography on a reversed-phase column. The chromatographic behavior of bile acid 3-N-acetylglucosaminides was dependent on the number and positions of hydroxyl groups and the structure of the side-chain. The bile acid 3-N-acetylglucosaminides were efficiently separated according to their conjugated form on a Cosmosil 5 C 18 column using a 0.3% potassium phosphate buffer-aceto-nitrile system. The chromatographic separation of ursodeoxycholate 3- and 7-N-acetylglucosaminides is also discussed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
