Abstract
1,2-Naphthoquinone-4-sulphonate has been used as a derivatising agent for a variety of primary and secondary aliphatic amines in dilute aqueous solutions prior to separation by reversed-phase high-performance liquid chromatography. Although the method is generally reliable, it does not work with some amines and this was explained in terms of steric hindrance during derivatisation and failure to transfer derivatives of hydrophilic amines to the organic phas during the extraction/concentration stage.
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