Abstract
The presnet status of the undeveloped branch of high-performance immobilized-metal-ion affinity chromatography (HPIMAC) is reviewed. As demonstrated, hgih resolution of peptides and protein mixtures can be obtained. Under specified conditions, more than 1000 runs can be made repeatedly on a single column without much change in pattern or recovery. Occasionally 10 3- to 10 4-fold purification of a protein can be obtained in a single chromatographic run.
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