High-performance hydrophobic interaction chromatography of proteins

Abstract

High-performance hydrophobic interaction chromatography of proteins was investigated on Butyl-G3000SW and Phenyl-G3000SW prepared by coupling butyl and phenyl groups with G3000SW, which is a support for high-performance gel chromatography. It was possible to separate proteins with high efficienty under mild eluting conditions such as isocratic elution with 0 to 2 M ammonium sulphate in 0.1 M phosphate buffer (pH 6.0) and linear gradient elution with ammonium sulphate concentration decreasing from 1.5 or 2 M to 0 in 0. 1 M phosphate buffer (pH 6.0). The recovery of proteins or enzymatic activity was almost quantitative.