Abstract
AbstractIn order to improve the nutritive value of rice protein, it is necessary to increase the level of lysine and threonine. Ten high‐lysine mutants were selected from N‐methyl‐N‐nitrosourea‐induced (MNU‐induced) mutant lines, using high‐performance liquid chromatography for amino acid analysis. The lysine content of these mutants ranged from 5.10% to 6.38% of total amino acids, while that of the original varieties was about 4%. All high‐lysine mutants also had a greatly increased histidine content and a floury endosperm. An increase in lysine content of the albumin/globulin fraction was characteristic of the MNU‐induced high‐lysine mutants. The segregation ratio of normal to floury endosperm in the F2 seeds from a cross between ‘Kinmaze’ and a high‐lysine mutant fitted the expected 3:1 ratio. The high level of lysine and the floury endosperm may be controlled by the same gene.
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