Highly stable and sensitive fluorescent probes (LysoProbes) for lysosomal labeling and tracking.

Nazmiye B Yapici,Megan Faucett,Jingfang Ju,Xin Chen,William J Pavan,Steffen Jockusch,Pengfei Li,Xin Yan,Lanrong Bi,Srinivas Rao Mandalapu,K Michael Gibson,Yue Bi

doi:10.1038/srep08576

Abstract

We report the design, synthesis and application of several new fluorescent probes (LysoProbes I-VI) that facilitate lysosomal pH monitoring and characterization of lysosome-dependent apoptosis. LysoProbes are superior to commercially available lysosome markers since the fluorescent signals are both stable and highly selective, and they will aid in characterization of lysosome morphology and trafficking. We predict that labeling of cancer cells and solid tumor tissues with LysoProbes will provide an important new tool for monitoring the role of lysosome trafficking in cancer invasion and metastasis.

Highlights

We report the design, synthesis and application of several new fluorescent probes (LysoProbes I-VI) that facilitate lysosomal pH monitoring and characterization of lysosome-dependent apoptosis
LysoProbes are superior to commercially available lysosome markers since the fluorescent signals are both stable and highly selective, and they will aid in characterization of lysosome morphology and trafficking
We predict that labeling of cancer cells and solid tumor tissues with LysoProbes will provide an important new tool for monitoring the role of lysosome trafficking in cancer invasion and metastasis

Summary

Labeling and Tracking

Synthesis and application of several new fluorescent probes (LysoProbes I-VI) that facilitate lysosomal pH monitoring and characterization of lysosome-dependent apoptosis. LysoProbes are superior to commercially available lysosome markers since the fluorescent signals are both stable and highly selective, and they will aid in characterization of lysosome morphology and trafficking. The second category of lysosomal markers are designed based upon endocytotic properties - the ability of large molecules to enter living cells. Along these lines, dextran and bovine serum albumin (BSA) labeled with a fluorophore are often used for endosome/lysosome labeling. We describe a new approach to highly sensitive and specific fluorophore labeling of lysosomes in the current report

Results and Discussion

Conclusions

Author contributions

Additional information