Highly Sensitive Voltammetric Determination Of Dantrolene Sodium In Pure Form, Pharmaceuticals, Human Breast Milk And Urine At Pencil Graphite And Glassy Carbon Electrodes

Abstract

Voltammetric behavior of dantrolene sodium (Dan) was comparatively investigated in Britton Robinson (BR) buffer as electrolyte at pencil graphite (PGE) and glassy carbon (GCE) electrodes, cyclic voltammetry (CV) gave one reversible cathodic anodic peak at -0.302, -0.249 V at PGE and -0.257, -0.178 V at GCE respectively and another irreversible cathodic one at -0.748 at PGE and -0.694 V at GCE. Differential pulse (DPV) and square wave (SWV) voltammetry were investigated for the reversible cathodic peak and gave at PGE, linearity range from 0.395-2.955 and 0.395-1.9 µg/mL with correlation coefficient 0.999 for both, LOD 0.09 and 0.052µg/mL, LOQ 0.273 and 0.158 µg/mL, respectively and at GCE, linearity range from 0.974- 2.44 and 0.199- 2.96 µg/mL with correlation coefficient 0.999 for both, LOD 0.167 and 0.095 µg/mL, LOQ 0.507 and 0.289 µg/mL, respectively. The proposed procedures were successfully applied to the determination of Dan with good recovery in pharmaceutical dosage form, human mother milk and urine directly without any pretreatment at both electrodes. The techniques were validated and revealed good accuracy and reproducible results. Keywords: Dantrolene Sodium, Biological fluids, pencil graphite electrode, glassy carbon electrode, Pharmaceuticals, Voltammetry.