Abstract
Microcantilever-based immunosensor is a next-generation electromechanical technique with broad application in biological detection. In this paper, we reported a microcantilever-based immunosensor that quantitatively detect the carbofuran, by using monoclonal antibodies to carbofuran as the receptor molecules. The surface of gold-coated microcantilever was chemically modified by the crosslinking of l-cysteine (l-cys)/glutaraldehyde (GA). The monoclonal antibodies to carbofuran were then immobilized on the side of the microcantilever to fabricate the immunosensor, the mechanical bending induced by antigen-antibody specific binding under an experimental environment. Under the optimized conditions, immunosensor detected carbofuran showed a good linear relationship over the range from 1.0×10-7 to 1.0×10-3g/L (R=0.998), with a detection limit of 0.1ng/mL. Moreover, the proposed immunosensor exhibited high sensitivity, specificity and good stability and can be successfully applied in the carbofuran determination in soil and vegetable samples with satisfactory results.
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