Abstract
AbstractThis paper describes the electrochemical determination of vitamin D2 (ergocalciferol) and D3 (cholecalciferol) in mixed organic/water solvent, using a glassy carbon electrode (GCE). The mixing ratio of organic/water solvent has an important influence on the electrocatalytic response of D vitamins on the surface of the glassy carbon electrode. Well‐defined peaks for Vitamin D2 and D3 were observed in a 40 % ethanol/60 % water solution with lithium perchlorate as the support electrolyte. This study demonstrated that the glassy carbon electrode is highly sensitive for the determination of vitamin D2 and D3, with a limit of detection of 0.13 and 0.118 µmol L−1, respectively. No significant interference was seen for vitamins A, E and K in the detection of vitamin D.
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