Highly sensitive determination of uric acid in the presence of major interferents using a conducting polymer film modified electrode

Abstract

This paper describes the sensitive and selective determination of uric acid (UA) in the presence of important interferences, ascorbic acid (AA), dopamine (DA), tyrosine (Tyr) and methionine (Met) at physiological pH using an electropolymerized film of 3-amino-5-mercapto-1,2,4-triazole on glassy carbon (p-AMTa) electrode. The p-AMTa electrode shows an excellent electrocatalytic activity towards UA. This was understood from the observed higher oxidation current and heterogeneous rate constant (3.24×10−5ms−1) for UA when compared to bare GC electrode (4.63×10−6ms−1). The selective determination of UA in the presence of 1000-fold excess of AA was achieved using p-AMTa electrode. Further, the p-AMTa electrode was successfully used for the simultaneous and selective determination of UA in the presence of important interferences, DA, Tyr and Met. Using amperometric method, 40nM UA was detected for the first time. The current response of UA was increased linearly while increasing its concentration from 40nM to 0.1mM and a detection limit was found to be 0.52nM (S/N=3). Finally, the practical application of the present method was demonstrated by determining UA in human urine and blood serum samples.