Abstract
An enzymeless biosensor, based on electrodeposition of overoxidized polypyrrole (OPPY) onto gold electrode and modified with titan yellow (TY), was investigated in this study. OPPY/TY/Au showed electrocatalytic activity for the oxidation of methyldopa (MDA) in acetate buffer solution. The electrochemical performance of the modified electrodes was investigated by differential pulse voltammetric (DPV) method. The resulting biosensor exhibited excellent performance for MDA determination with a wide linear range (50nM to 20μM), a highly reproducible response (RSD of 2.3%), low percentage of the interferences and long-term stability. The calculated detection limit was 17nM. In order to verify the reliability of the biosensor, it was applied to the determination of MDA in human serum and pharmaceutical samples.
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