Abstract
BackgroundAs a promising candidate for artificial enzymes, catalytically active nanomaterials show several advantages over natural enzymes, such as controlled synthesis at low cost, tunability of catalytic activities, and high stability under stringent conditions. Rod-shaped Au–Pt core/shell nanoparticles (Au@Pt NRs), prepared by Au nanorod-mediated growth, exhibit peroxidase-like activities and could serve as an inexpensive replacement for horseradish peroxidase, with potential applications in various bio-detections. The determination of measles virus is accomplished by a capture-enzyme-linked immunosorbent assay (ELISA) using Au@Pt NR-antigen conjugates.ResultsBased on the enhanced catalytic properties of this nanozyme probe, a linear response was observed up to 10 ng/mL measles IgM antibodies in human serum, which is 1000 times more sensitive than commercial ELISA.ConclusionsHence, these findings provide positive proof of concept for the potential of Au@Pt NR-antigen conjugates in the development of colorimetric biosensors that are simple, robust, and cost-effective.
Highlights
As a promising candidate for artificial enzymes, catalytically active nanomaterials show several advantages over natural enzymes, such as controlled synthesis at low cost, tunability of catalytic activities, and high stability under stringent conditions
Characterization of Au@Pt NRs and Au@Pt NR‐antigen conjugates Au NRs were employed as templates to guide the growth of Pt
The longitudinal surface plasmonic resonance (SPR) band shows a quite large redshift (100 nm) with slight damping in the intensity and an evident broadening in the width
Summary
As a promising candidate for artificial enzymes, catalytically active nanomaterials show several advantages over natural enzymes, such as controlled synthesis at low cost, tunability of catalytic activities, and high stability under stringent conditions. Enzyme-linked immunosorbent assay (ELISA) is the most widely accepted and powerful method for virus detection. This method commonly uses a horseradish peroxidase (HRP)-labeled immunoreagents to realize the amplification of detection signals and the identification of target molecules [2]. Nanozymes are advantageous in several aspects, such as low cost, ease of mass production, robustness in harsh environments, high stability, long-term storage and large surface area for further modification and bioconjugation [10]. Combination of biomolecules with NPs provides interesting tools for improvements in traditional ELISA [12, 13]
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