Abstract

Mutagenic formaldehyde (FA) is usually abused to preserve seafood, thus it is very necessary to detect harmful formaldehyde in seafood with a simple and effective method. In this work, we facilely prepared a new fluorescent probe RBNA, which showed a remarkable fluorescence lighting-up response towards FA with high sensitivity and selectivity, fast response (within 5 min) and a low detection limit (0.21 μM). The fluorescence intensity is linearly related to the concentration of FA ranging from 0 to 120 μM (R2 = 0.9952), which enables it to quantitatively determine the concentration of FA. This probe was successfully used to detect FA in seafood samples with good recoveries (80-119%). Furthermore, the probe has been utilized to image FA in living cells and zebrafish with good performance. Therefore, this probe has a good capability for rapid and sensitive determination of FA in seafood.

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