Abstract
To achieve simple and efficient separation of acteoside (ACT), extraction ACT from crude extracts of Cistanche tubulosa via ionic liquid (IL) based aqueous two-phase system (ATPS) is investigated. The IL–ATPS comprising [C4mim]BF4 and (NH4)2SO4 exhibits excellent extraction performance, extracting almost all ACT in the [C4mim]BF4-rich phase. The high polarity of [C4mim]BF4 having multi-hydrogen bond receptors is conducive for extracting ACT in the IL-rich phase, and (NH4)2SO4 not only provides a weak acidic microenvironment for increasing ACT stability in the IL-rich phase but also reduces the solubility of ACT in the salt-rich phase via the salting-out effect. In addition, molecular simulation results show that ACT is extracted in the [C4mim]BF4-rich phase via multi-interactions, including hydrogen bonding, van der Waals forces, and π–π stacking. This study is expected to provide a valuable reference for the separation of bioactive constituents from natural products.
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