Abstract
Two highly repetitive sequences were isolated from Bam HI — based partial genomic library of pearl millet [Pennisetum glaucum (L). R Br]. Time — course digests of pearl millet genomic DNA on hybridization with one of the clones (PGB 625) revealed a ladder of fragments with a ∼ 0.13 kb repeat length while the sequences hybridizing with the other clone (PGB 662) revealed complex organization in the genome. Isoschizomer cleavage demonstrated the absence of methylation at GATC sites in tandem arrays and extensive sequence changes in repeating motifs. Southern blot hybridizations of genomic DNA restriction fragments from five Pennisetum species and seven other cereals with PGB 625 and PGB 662 revealed the nature and extent of sequence variation. Abundance of PGB 625 related sequences in P. purpureum (an allotetraploid) was comparable to that of P. glaucum whereas the abundance of sequences related to the other clone (PGB 662) was very low suggesting differential modulation in the progenitor genome following polyploidization. Signal intensities of the sequences related to these two inserts were low in other Pennisetum species and cereals, indicative of their low copy nature. Thus, these clones can be used as genome — specific probes in tracking genomic changes in wide hybrids and in expanding cereal genome mapping.
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