Highly Ozonated Sunflower Oil Dose May Cause Oxidative Stress and Impact the Quality of Life After Palatal Graft Removal: Secondary Endpoints of a Randomized Controlled Clinical Trial

Abstract

ABSTRACT The aim of this study was to evaluate the effect of highly ozonated sunflower oil on postoperative pain and quality of life in patients undergoing removal of a free gingival graft (FGG) in the donor area of the palate. After graft removal, the palatal wound was treated with highly ozonated sunflower oil with a peroxide index between 510 and 625 meq/kg in the test group (n = 14) and with non-ozonated sunflower oil in the control group (n = 14). These are the secondary endpoints of a randomized clinical trial. Questionnaires assessing oral health impact on quality of life (OHIP-14) and postoperative pain (VAS scale) were applied to all participants at day 7. The results did not reveal a statistically significant difference in the postoperative pain between groups in all periods (P>0.05). There was a significant positive correlation in both groups for pain and amount of analgesic consumption (P<0.0001). It was found that participants in the test group showed a higher overallscore for OHIP-14 than the control group (P < 0.05), with impact on unsatisfactory diet, meal interruptions and irritability (P < 0.05). MDA levels measured 7 days post-treatment were higher in the test group compared to those of the control group (P < 0.05). It can be concluded that high dose and overapplication of the ozonated sunflower oil had a negative impact on the quality of life of individuals after palatal graft removal, which might have been aggravated by oxidative stress. Trial Registration number NCT05620550/Date of registration: November 16, 2022