Abstract
Highly blue luminescent nitrogen-doped carbon dots (N-CDs) with a fluorescence quantum yield of 42.3% were prepared by an efficient one-step pyrolytic route from ethylenediaminetetraacetic acid and urea. The as-synthesized N-CDs were demonstrated as an effective fluorescent probe for label-free, selective and sensitive recognition of Fe3+ with a linear range of 0.5μM to 2mM and a detection limit of 13.6nM due to Fe3+-quenched fluorescence (turn-off). The quenched fluorescence could be turned on after the addition of apoferritin owing to the removal of ferric species from the surface of N-CDs by apoferritin, making complex N-CDs/Fe3+ a selective apoferritin probe with a linear range of 0.1–25μM and a detection limit as low as 2.6nM. In addition, the application of this novel N-CDs-based probe for imaging Fe3+ ions and apoferritin in living cells suggest that this sensing system has great potential applications in biosensing, bioimaging, and many other fields.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have