Abstract
By using paraffin liquid and oleic acid as the solvent and ligand, highly-fluorescent red to near-infrared (NIR)-emitting cadmium selenium telluride (CdSe1−xTex) alloyed quantum dots (QDs) with photoluminescence quantum yield (PLQY) up to 70% were successfully prepared. The as-prepared CdSe1−xTex QDs also demonstrated good optical stabilities against the temperature change and photobleaching due to their gradient “CdTe-rich core/CdSe-rich surface” quasi “core/shell” structure, which was confirmed by studying the alloying process of CdSe1−xTex QDs. Moreover, the as-prepared red to NIR CdSe1−xTex QDs were successfully incorporated into the carboxyl capped porous polystyrene (PS) microbeads to fabricate red to NIR-emitting microbeads with low in vitro cytotoxicity, and the biotinylated goat anti-mouse IgG could be specifically coupled to the optical encoded beads via mouse anti-human chorionic gonadotrophin connected to the carboxyl groups on the encoded microbeads successfully, which suggested promising application of CdSe1−xTex QDs in multiplexed bioassays.
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