Abstract
Gene expression within human glioblastomas were analyzed from data on 20,083 genes entered into the on-line Human Protein Atlas. In selecting genes that are strongly expressed within normal human brain tissue, 58 genes were identified from a search of the 20,083 entries that were rated as showing 90% or greater intensity of expression within normal brain tissues. Of these 58, a subset of 48 genes was identified that not only had expression data for human glioblastomas but also for the human glioblastoma cell line U-251. Four of these 48 selected genes were found to be strongly expressed within the cytoplasm when assessed by both histologic sampling of high grade glioma patient cases as well as U-251 glioblastoma cell line immunofluoresence analysis. These four human genes are: AGBL2 (ATP/GTP binding protein-like 2), BLOC1S6 (biogenesis of lysosomal organelles complex-1, subunit 6), MAP1A (microtubule-associated protein 1A) and ZSWIM5 (zinc finger, SWIM-type containing 5, also known as KIAA1511). Further research is advocated to investigate the role of ZSWIM5 and AGBL2 in glioma cell biology.
Highlights
Acknowledgements: the author wishes to thank Dr Janet Bay for reviewing the initial version of this manuscript chemistry and immunofluoresence as the Conflict of interests: the author declares no basis for evaluation from diverse clinical cases potential conflict of interests
Gene expression within human glioblastomas were analyzed from data on 20,083 genes entered into the on-line Human Protein Atlas
Of these 58, a subset of 48 genes was identified that had expression data e for human glioblastomas and for the human s glioblastoma cell line U-251. Four of these 48 u selected genes were found to be strongly l expressed within the cytoplasm when assessed ia by both histologic sampling of high grade glioma patient cases as well as U-251 glioblasc toma cell line immunofluoresence analysis. r These four human genes are: AGBL2 (ATP/GTP e binding protein-like 2), BLOC1S6, m MAP1A
Summary
Acknowledgements: the author wishes to thank Dr Janet Bay for reviewing the initial version of this manuscript chemistry and immunofluoresence as the Conflict of interests: the author declares no basis for evaluation from diverse clinical cases potential conflict of interests. Four of these 48 u selected genes were found to be strongly l expressed within the cytoplasm when assessed ia by both histologic sampling of high grade glioma patient cases as well as U-251 glioblasc toma cell line immunofluoresence analysis.
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