Highly efficient solid-phase phosphopeptide synthesis using bis-(polyfluorophenyl) chlorophosphates: Preparation of serine-threonine protein phosphatase substrates

Abstract

A new fast and efficient solid phase phosphorylation protocol suitable for the preparation of phosphoserine- and phosphothreonine-containing peptides is described. The method allows the facile conversion of primary and secondary alcohol groups to the corresponding bis-(pentafluorophenyl) phosphate triesters which are cleanly converted to the phosphate monoesters under acidic conditions.