Highly efficient microbial lipid synthesis from co-fermentation of enzymatic hydrolysate of sugarcane bagasse by a Trichosporon dermatis mutant

Abstract

In this study, an oleaginous yeast Trichosporon dermatis was mutated by a sequential plasma and chemical mutagenesis approach. The best mutant (L7) was investigated to produce lipid from enzymatic hydrolysates derived from pretreated sugarcane bagasse at different scales and cultivation modes. The results showed that in shake flasks, L7 produced 26.7 g/L cell mass with 14.0 g/L lipid from the hydrolysate medium containing 80 g/L glucose/xylose (mass ratio of 2:1) in batch mode, and 34.9 g/L cell mass with 20.2 g/L lipid from the medium containing a total of 120 g/L glucose/xylose (2:1) in fed-batch mode, respectively. In a 5 L fermenter, L7 produced 33.3 g/L cell mass with 18.1 g/L lipid in batch mode and 51.5 g/L cell mass with 31.3 g/L lipid in fed-batch mode, respectively. This study demonstrates that L7 is highly efficient T. dermatis strain to produce lipid using glucose and xylose mixture derived from lignocellulose.