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Abstract
Abstract Branched oligodeoxyribonucleotides (ODNs) are key molecules in constructing nano-scale structures such as DNA lattices, dendrimer-like DNA as well as DNA devices including DNA walkers, DNA memory, and DNA computer. In this paper, two types of highly efficient methods were used to construct the comb-like DNA by using template-directed reversible DNA photocrosslinking via 5-(2-carbamoylvinyl)-2′-deoxyuridine (cvU). The efficiency and thermodynamic stability of three types of reversible DNA photocrosslinked, linear ODN, single-branched ODN (5′-end) and single-branched ODN (mid) reported previously, were also compared. The results show that the most efficient one of the three is a single-branched ODN (5′-end), despite its low thermodynamic stability. In addition, we also showed that this reversible photocrosslinking method is applicable to nanotechnology by constructing comb-like DNA. This method should have great potential in nanotechnology by serving as a platform for fabrication and synthesis, because their sizes and structures can be changed through the alteration of building blocks. We believe that this method is versatile and may find a myriad of applications in the nano-biotechnology field.
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