Highly efficient formation of droplet interface bilayers by using a microperforated separator

Abstract

This study reports an efficient droplet interface bilayer (DIB) formation using an open chamber with an external separator that can divide an aqueous solution to two regions. This approach facilitates reagent-saving and simplification of the device fabrication. Amphiphobic modification of the separator surface enables us to handle the device easily without liquid spill, and assists bilayer lipid membrane (BLM) formation. Furthermore, we found that pretreatment of lipid deposition on the separator increased the success rate of BLM formation. We are assured of this method's availability for various research and development fields based on DIB applications.